Lipid synthesis: where chemical synthesis meets lipid analysis.

Monday, April 29, 2024

5:00 PM – 6:00 PM EDT

Presenting Author(s)

Jonas Blockx, PhD

Dr.
KU Leuven KULAK
Kortrijk, West-Vlaanderen, Belgium

Co-Author(s)

EV

Eline Van Wayenbergh

Dr. Ir.
KU Leuven, Belgium
CC

Christophe Courtin

Prof. Dr. Ir.
KU Leuven, Belgium
WT

Wim Thielemans

Prof. Dr. Ir.
KU Leuven KULAK, Belgium
IF

Imogen Foubert

Prof. Dr. Ir.
KU Leuven KULAK, Belgium

Abstract: The complexity of lipids makes analyzing them a challenge and depending on the goal of the analysis, different techniques can be used. For most of these techniques, lipid standards are required for accurate measurements. These standards should, in the ideal case, be comparable to the analyte. Lipid standards are often not (sufficiently) commercially available, or are unsuitable as internal standards due to their presence in the sample of interest. Relative simple chemical synthesis of lipids or derivatives thereof could already solve part of the problem. As examples, two case studies will be discussed: on the one hand the synthesis of a N-acyl phosphatidyl ethanolamine (NAPE) and its derivative, a N-acyl ethanolamine (NAE), for the use as internal standard in the study of lipase activity in a fermented food product using LC-MS as analysis technique. On the other hand the synthesis of the lipid derivative retinyl tridecanoate and its use in the study of retinyl palmitate stability.