Effect of in vivo modulation of microbial genome synthase on phyto-compounds towards drug discovery

Overview: Biocatalyst hydrolytic function, socio-economic importance and industrial relevance were identified in this investigation and the research aimed to determine the effect of microbial enzyme infusion on phyto-compounds yield and antimicrobial expression.

Methodology: The following activities were carried out to achieve the objective: i. construct of a hyperactive Bacillus subtilis sub-specie using a modulation kit, ii. production of amylase, cellulase and pectinase using the successfully modulated strain, iii. Applying a consortium of the enzymes to infuse powdery samples of Calotropis procera Ait Leaf under synthase proximate conditions of pH 6 at 60^oC for 1 hour, and iv. analysis on extract to ascertain phyto-compound yield and antimicrobial expression.

Results: The following results were recorded: i. infused extract with a consortium of catalysts showed a high impact of 35% increment in secretion of terpenoids, flavonoids, anthraquinone, alkaloids, steroids, tannins, and saponins that were analysed and identified to be present compared to extracts of dimethyl ether, 70% ethanol, and methanol; ii. results also recorded the high impact of bioactive agent expression against test strains with the following photo-restraint articulations of 33.3mm, 28.6 mm, 30.4 mm, 31.6 mm, 30.6 mm, and 22.3 mm for Escherichia coli, Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, and Candida albicans respectively against concentrates of dimethyl ether, 70% ethanol, and methanol used as controls. The outcome of this investigation demonstrated the significance of enzyme application in drug discovery toward alleviating challenges from pathogens causing progressive diseases that are influencing healthcare and overall environmental declination.