Robust quantification capabilities for the analysis of di- and triglycerides in edible fats and oils

Triacylglycerols (triglycerides, TAG) with lower amounts of diacylglycerols (diglycerides, DAG), monoacylglycerols (monoglycerides, MAG) are the primary constituents of edible oils and fats, and accordingly define their quality. Typically, those main constituents are quantified in a single number, but are in fact consisting of many compounds. A full analysis of the TAG molecular species is demanding a different approach because of the different types of molecular species available.

In this work, the authors will show the fruits of the combined efforts of several Cargill R&D teams across the globe on this topic. Both GC and LC based analytical solutions allow us the precise identification and quantification of TAG and DAG in a broad spectrum of specialty edible oils and fats. Our GC-FID capability comprises a derivatization step together with the use of correction factors, and offers the successful quantifications of about 50 TAG molecular species in edible oils. Our UHPLC-CAD capability provides a ‘dilute and shoot’ sample preparation of fats and oils, with a quantification based on an external calibration curve and internal standards. A detailed comparison of the two approaches will be presented.

These analytical capabilities are the keystones to support new product and process developments in the edible oils and fats industry and beyond.